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Updated: May 7, 2026

Development of Leishmania Species Strains with Constitutive Expression of eGFP
Published on: April 21, 2023
Constitutive mosaic aneuploidy is a unique genetic feature widespread in the Leishmania genus
Laurence Lachaud1, Nathalie Bourgeois2, Nada Kuk3
1University Montpellier 1, Faculty of Medicine, Laboratory of Parasitology-Mycology, Montpellier, France; CNRS 5290, IRD 224, University Montpellier 1&2 (UMR "MiVEGEC"), Montpellier, France.
Abstract:
Using fluorescence in situ hybridization, we determined the ploidy of four species of Leishmania: Leishmania infantum, Leishmania donovani, Leishmania tropica and Leishmania amazonensis. We found that each cell in a strain possesses a combination of mono-, di- and trisomies for all chromosomes; ploidy patterns were different among all strains/species. These results extend those we previously described in Leishmania major, demonstrating that mosaic aneuploidy is a genetic feature widespread to the Leishmania genus. In addition to the genetic consequences induced by this mosaicism, the apparent absence of alternation between haploid/diploid stages questions the modality of genetic exchange in Leishmania sp.
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