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Updated: May 7, 2026

Gene Trapping Using Gal4 in Zebrafish

Gene Trapping Using Gal4 in Zebrafish

Published on: September 29, 2013

Gene trapping using gal4 in zebrafish.

Jorune Balciuniene¹, Darius Balciunas

¹Department of Biology, Temple University.

Journal of Visualized Experiments : Jove

|October 15, 2013

Summary

This summary is machine-generated.

This study enhances zebrafish gene trapping by modifying a Gene Breaking Transposon (GBT) vector. The new vector uses Gal4-VP16 for sensitive gene trap detection and creating specific transgenic lines.

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Last Updated: May 7, 2026

Gene Trapping Using Gal4 in Zebrafish

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Published on: September 29, 2013

Targeting Olfactory Bulb Neurons Using Combined In Vivo Electroporation and Gal4-Based Enhancer Trap Zebrafish Lines

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Live Imaging of Innate Immune and Preneoplastic Cell Interactions Using an Inducible Gal4/UAS Expression System in Larval Zebrafish Skin

Live Imaging of Innate Immune and Preneoplastic Cell Interactions Using an Inducible Gal4/UAS Expression System in Larval Zebrafish Skin

Published on: February 3, 2015

Area of Science:

Genetics
Molecular Biology
Developmental Biology

Background:

Zebrafish are a key vertebrate model for insertional mutagenesis due to large clutch size and transparent embryos.
Previous zebrafish gene trap vectors had limitations in stringency and mutagenicity.
Gene Breaking Transposons (GBTs) were developed to overcome these limitations.

Purpose of the Study:

To modify the GBT-R15 vector for improved gene trapping in zebrafish.
To incorporate Gal4-VP16 as a primary reporter for enhanced sensitivity and utility.
To add UAS:eGFP as a secondary reporter for direct gene trap event detection.

Main Methods:

Modification of the GBT-R15 transposon vector.
Introduction of Gal4-VP16 as the primary gene trap reporter.
Addition of a UAS:eGFP secondary reporter for direct visualization.

Main Results:

The modified vector increases sensitivity for trapping genes with low expression levels.
Gene trap lines can be used as Gal4 drivers for targeted transgene expression.
The system successfully identified mutations in genes with diverse cellular functions (nuclear, cytoplasmic, membrane).

Conclusions:

The enhanced GBT vector improves zebrafish insertional mutagenesis efficiency and versatility.
Gal4-VP16 reporter system offers advantages for studying gene function, especially for non-essential genes.
This tool facilitates the generation of specific transgenic zebrafish lines for various research applications.