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[Research on HPLC fingerprint of Rheum palmatum from Gansu Province called "quan-shui-da-huang"]
Zheng-Rui Niu1, Hong-Yu Liu, Qing-Sheng Zhang
1Institute for Food and Cosmetics Control, National Institutes for Food and Drug Control, Beijing 100050, China.
Objective:
To establish an analysis method for HPLC fingerprint of Rheum palmatum collected from Gansu province (called "Quan-shui-da-huang"), and provide basis for quality control of Quan-shui-da-huang.
Methods:
The Cosmosil 5C18-PAQ column (250 mm x 4.6 mm, 5 microm) was used with the mixture of 0.1% acetic acid and acetonitrile as mobile phase in a gradient elution mode. The flow rate was 1 mL/min, column temperature was 30 degrees C and detection wavelength was set at 280 nm. The similarity evaluation, principal component analysis (PCA) and cluster analysis of crude drugs collected from different habitats were carried out.
Results:
The analysis method of HPLC fingerprint was set up. The characteristic fingerprint was obtained by chemometrics methods.
Conclusion:
Under the selected chromatographic conditions, constituents in Rheum palmatum can be separated well. This method can be used for the quality control of Quan-shui-da-huang.
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