[Study on chemical components in yiqing capsule based on UPLC-ESI-MS-MS and FTIR and its anti-inflammatory activity
1Guangdong College of Pharmacy, Guangzhou 510006, China. luolan1105@126.com
Objective:
To study the chemical components in Yiqing capsule by UPLC-ESI-MS-MS and its anti-inflammatory activity in vitro.
Methods:
The mass spectrometer equipped with an eletrospray ion source in positive ion mode was used and data of mass spectrum and mass/mass spectrum were analyzed; FTIR was used to detect the characteristic spectra of ten bcathes of Yiqing capsule. Compared the spectra by principal component analysis and cluster analysis; RAW264.7 cell model induced by LPS was established to study the inhibitory effect of Yiqing capsule on the release of NO.
Results:
Totally twelve chemical components were analyzed by the established UPLC-ESI-MS-MS method; The results of the principal component analysis and the cluster analysis indicated that there were significant differences between the sample (batch number: 120703) and other different batches of samples; Yiqing capsule could inhibit the release of NO induced by LPS in a dose-dependent manner at the concentration of 1 - 100 micromol/L.
Conclusion:
UPLC-ESI-MS-MS and FTIR which are rapid, convenient and effective, can be used for quality control of Yiping capsule; Yiqing capsule exhibites anti-inflammatory activity in vitro.
