Related Experiment Video
Updated: May 6, 2026
Chemo-enzymatic Synthesis of N-glycans for Array Development and HIV Antibody Profiling
Published on: February 5, 2018
Chemoenzymatic synthesis of the human CD52 and CD24 antigen analogues
Zhimeng Wu1, Xueqing Guo, Guofeng Gu
1Department of Chemistry, Wayne State University , Detroit, Michigan 48202, United States , and National Glycoengineering Research Center, Shandong University , Jinan 250100, China.
Abstract:
Analogs of the human CD52 and CD24 antigens carrying the common core structure of glycosylphosphatidylinositol (GPI) anchors and the intact polypeptide sequences of CD52 and CD24 were chemoenzymatically synthesized. CD52 and CD24 proteins were obtained by solid-phase peptide synthesis and then coupled to chemically synthesized GPI anchors under the influence of a bacterial enzyme, sortase A, to afford the target molecules in good yields.
