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Distinguishing hygroma and amniotic fluid
Prenatal Diagnosis
|September 1, 1985
Summary
Alkaline phosphatase (ALP) isoenzyme analysis differentiates cystic hygroma fluid from amniotic fluid. Hygroma fluid shows serum-type ALP, while amniotic fluid is rich in intestinal isoenzymes.
Area of Science:
- Biochemistry
- Prenatal Diagnostics
- Medical Laboratory Science
Background:
- Cystic hygromata are congenital lymphatic malformations.
- Distinguishing cystic hygroma fluid from amniotic fluid is crucial for diagnosis and management.
- Alkaline phosphatase (ALP) is an enzyme with multiple isoenzymes.
Purpose of the Study:
- To establish a biochemical method for differentiating fluid from cystic hygromata and amniotic fluid.
- To investigate the isoenzyme profile of alkaline phosphatase in both fluid types.
Main Methods:
- Measurement of alkaline phosphatase (ALP) isoenzymes in fluid samples.
- Analysis of the relative proportions of liver/bone/kidney and intestinal isoenzymes.
Main Results:
- Fluid from cystic hygromata exhibited a serum-type ALP profile, predominantly liver/bone/kidney isoenzyme.
- Second-trimester amniotic fluid ALP contained a high proportion (approximately 80%) of intestinal isoenzyme.
- A clear distinction in ALP isoenzyme patterns was observed between the two fluid types.
Conclusions:
- Alkaline phosphatase isoenzyme analysis is a reliable method to differentiate cystic hygroma fluid from amniotic fluid.
- The distinct isoenzyme profiles provide a biochemical basis for diagnosis in prenatal settings.