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Updated: May 6, 2026

Sequencing of mRNA from Whole Blood using Nanopore Sequencing
Published on: June 3, 2019
Nanopore-based identification of individual nucleotides for direct RNA sequencing
Mariam Ayub1, Steven W Hardwick, Ben F Luisi
1Department of Chemistry, University of Oxford , Oxford OX1 3TA, United Kingdom.
Abstract:
We describe a label-free ribobase identification method, which uses ionic current measurement to resolve ribonucleoside monophosphates or diphosphates in α-hemolysin protein nanopores containing amino-cyclodextrin adapters. The accuracy of base identification is further investigated through the use of a guanidino-modified adapter. On the basis of these findings, an exosequencing approach is envisioned in which a processive exoribonuclease (polynucleotide phosphorylase) presents sequentially cleaved ribonucleoside diphosphates to a nanopore.
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