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Updated: May 6, 2026
Multi-target Chromogenic Whole-mount In Situ Hybridization for Comparing Gene Expression Domains in Drosophila Embryos
Published on: January 31, 2016
Multicolour whole-mount in situ hybridization to Drosophila embryos
1Biozentrum der Universität Basel, Abteilung Zellbiologie, Klingelbergstrasse 70, CH-4056 Basel, Switzerland, , , , , , CH.
Abstract:
We report an extended whole-mount in situ hybridization procedure for Drosophila embryos. By using probes labelled with digoxigenin, fluorescein and biotin, respectively, this protocol allows the detection in three colours of RNAs derived from three different genes. Hybridized probes are detected by consecutive staining with appropriate alkaline phosphatase conjugates using different chromogenic substrate combinations, and serial removal of the antibody conjugates by low pH washes.
