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Proteomics

Proteomics

A proteome is the entire set of proteins that a cell type produces. We can study proteomes using the knowledge of genomes because genes code for mRNAs, and the mRNAs encode proteins. Although mRNA analysis is a step in the right direction, not all mRNAs are translated into proteins.
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term...

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Related Experiment Video

Updated: May 6, 2026

Isolation and Culture of Human Mature Adipocytes Using Membrane Mature Adipocyte Aggregate Cultures MAAC

Isolation and Culture of Human Mature Adipocytes Using Membrane Mature Adipocyte Aggregate Cultures MAAC

Published on: February 13, 2020

Comprehensive proteomic data sets for studying adipocyte-macrophage cell-cell communication.

Anja Freiwald¹, Christopher Weidner, Annabell Witzke

¹Otto Warburg Laboratory, Max Planck Institute for Molecular Genetics, Berlin, Germany

|November 1, 2013

Summary

Investigating adipocyte-macrophage interactions reveals key cellular communication pathways. This study identifies proteins and phosphorylation sites involved in cellular stress and inflammation, offering insights into metabolic diseases like type 2 diabetes.

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Last Updated: May 6, 2026

Isolation and Culture of Human Mature Adipocytes Using Membrane Mature Adipocyte Aggregate Cultures MAAC

Isolation and Culture of Human Mature Adipocytes Using Membrane Mature Adipocyte Aggregate Cultures MAAC

Published on: February 13, 2020

Proteomic Profiling of Macrophages by 2D Electrophoresis

Proteomic Profiling of Macrophages by 2D Electrophoresis

Published on: November 4, 2014

Adipocyte-Specific ATAC-Seq with Adipose Tissues Using Fluorescence-Activated Nucleus Sorting

Adipocyte-Specific ATAC-Seq with Adipose Tissues Using Fluorescence-Activated Nucleus Sorting

Published on: March 17, 2023

Area of Science:

Cell Biology
Metabolic Disease Research
Proteomics

Background:

Cellular communication is vital for biological processes.
Adipocyte-macrophage interactions are critical in developing diseases like type 2 diabetes.

Purpose of the Study:

To investigate the paracrine interactions between murine adipocytes and macrophages.
To identify proteins and phosphorylation sites involved in adipocyte-macrophage crosstalk.
To understand the molecular pathways contributing to metabolic impairment.

Main Methods:

Utilized a bilayer cell co-culture system.
Performed comprehensive mass spectrometry for proteome-wide analysis.
Analyzed protein and phosphopeptide data using enrichment and pathway analyses.

Main Results:

Identified 4486 proteins, with 2392 informative for adipocytes and 2957 for macrophages.
Detected over 12,000 phosphorylation sites, assigning 3,200 phosphopeptides for adipocytes and 4,514 for macrophages.
Deciphered regulatory pathways in cellular stress and inflammation linked to metabolic disorders.

Conclusions:

The study provides a molecular, pathway-centric view of adipocyte-macrophage interplay.
Identified key signaling pathways contributing to insulin resistance and other metabolic disorders.
Generated valuable datasets for future research on cellular communication in disease.