Related Experiment Video
Updated: May 6, 2026
Electrochemical Detection of Deuterium Kinetic Isotope Effect on Extracellular Electron Transport in Shewanella oneidensis MR-1
Published on: April 16, 2018
Estimation ofEscherichia coli mortality in seawater by the decrease in(3)H-label and electron transport system
J Martinez1, J Garcia-Lara, J Vives-Rego
1Departament de Microbiologia, Universitat de Barcelona, Av. Diagonal, 645, 08028, Barcelona, Spain.
Abstract:
The mortality ofEscherichia coli in seawater was assessed by viable counts, electron transport system activity, and cellular(3)H-labelling. Filtration was used to assess the grazing mortality. Cellular radiolabelling and electron transport system activity were useful methods for assessingE. coli survival in seawater. The decrease in the(3)H-label as a method to assess bacterial mortality was validated by using viable counts and metabolic activity assays. The particulate fraction that passed 2 μm but was retained on 0.2 μm pore-size filters was the primary reason forE. coli mortality in seawater.
