[Recombinant human IL-16 inhibits proliferation and induces apoptosis in MT-4 cells]
Moli Zhu1, Haiyan Song, Guizeng Zhao
1Research Center for Immunology, Xinxiang Medical University, Xinxiang 453003, China.
Objective:
To investigate the effects of recombinant human IL-16 (rhIL-16) on the proliferation and apoptosis of MT-4 cells.
Methods:
MT-4 cells were infected with adenovirus expressing rhIL-16 at different time points. Then the proliferation of MT-4 cells was measured by the MTT assay. The apoptosis of the MT-4 cells was evaluated by flow cytometry. The expression of two molecular markers for apoptosis, Bax and Bcl-2, were analyzed by Western blotting.
Results:
The MTT assay results showed that pAd-IL-16 transfectants had inhibitory effect on the proliferation of MT-4 cells, compared with untreated cells at various time points. Flow cytometry revealed that apoptotic rate of MT-4 cells was higher than that of control group. Furthermore, Western blotting indicated that the Bax protein expression increased while Bcl-2 protein expression decreased in a time-dependent manner in MT-4 cells infected with the rhIL-17-expressing adenovirus.
Conclusion:
These results demonstrated that IL-16 plays an important role in the proliferation and apoptosis of MT-4 cells, and the molecular mechanism underlying this phenomenon may involve the modulation of Bax and Bcl-2 expression.
Insights
Recombinant human interleukin-16 (rhIL-16) inhibits MT-4 cell proliferation and promotes apoptosis. This effect is linked to altered expression of apoptosis-related proteins Bax and Bcl-2.
Area of Science:
- Cell biology
- Molecular biology
- Immunology
Context:
- Interleukin-16 (IL-16) is a cytokine with diverse biological functions.
- MT-4 cells are a human T-cell line often used in virology and immunology research.
- Understanding IL-16's role in cell proliferation and apoptosis is crucial for various biological processes.
Purpose:
- To investigate the effects of recombinant human IL-16 (rhIL-16) on MT-4 cell proliferation.
- To determine the impact of rhIL-16 on apoptosis in MT-4 cells.
- To elucidate the molecular mechanisms, specifically the role of Bax and Bcl-2 proteins, in IL-16-mediated apoptosis.
Summary:
- MT-4 cells were infected with adenovirus expressing rhIL-16.
- Cell proliferation was assessed using MTT assays, while apoptosis was evaluated by flow cytometry.
- Western blotting was employed to analyze the expression levels of Bax and Bcl-2 proteins.
Impact:
- rhIL-16 significantly inhibited MT-4 cell proliferation.
- Apoptotic rates in MT-4 cells were elevated following rhIL-16 treatment.
- rhIL-16 induced a time-dependent increase in Bax expression and a decrease in Bcl-2 expression, suggesting a role in apoptosis regulation.
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