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Related Concept Videos

Preparation of Samples for Electron Microscopy01:20

Preparation of Samples for Electron Microscopy

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To be visualized by an electron microscope, either transmission or scanning, biological samples need to be fixed (stabilized) so the electron beam does not destroy them and dried thoroughly (desiccated/dehydrated) so the vacuum does not affect them. Fixation needs to be done as quickly as possible because the sample properties will start changing as soon as it is removed from its natural environment. For example, in a tissue sample, the oxygen levels begin decreasing, causing an altered...
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Using a Chemical Biopsy for Graft Quality Assessment
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Solid phase microextraction fills the gap in tissue sampling protocols.

Barbara Bojko1, Krzysztof Gorynski, German Augusto Gomez-Rios

  • 1Department of Chemistry, University of Waterloo, Waterloo, ON, Canada.

Analytica Chimica Acta
|November 13, 2013
PubMed
Summary
This summary is machine-generated.

Solid phase microextraction streamlines in vivo tissue analysis for metabolomics. This method simplifies sample collection and preparation, overcoming key bottlenecks in biomarker discovery during transplantation studies.

Keywords:
In vivo SPMEMetabolomicsSurgeryTissue sampling

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Area of Science:

  • Bioanalysis
  • Metabolomics
  • Biomarker Discovery

Background:

  • Untargeted tissue analysis is crucial for bioanalysis and biomarker discovery.
  • Current methods face challenges due to invasive sampling and complex sample preparation.
  • These limitations hinder the efficiency of metabolomics studies.

Purpose of the Study:

  • To present and evaluate a novel technology for in vivo sampling, sample preparation, and global metabolite extraction.
  • To address the bottlenecks associated with traditional untargeted tissue analysis.
  • To demonstrate the applicability of the developed method in transplantation models.

Main Methods:

  • Solid phase microextraction (SPME) technology was integrated for in vivo sampling and metabolite extraction.
  • The method was evaluated in a pig model during liver and lung transplantation.
  • Key sampling parameters including probe selection, transport, storage, and analyte coverage were discussed.

Main Results:

  • The study presented an integrated approach for in vivo sample collection and metabolite extraction.
  • The solid phase microextraction method was successfully applied and evaluated in a relevant animal model.
  • The developed technique demonstrated applicability for metabolomics studies in the context of transplantation.

Conclusions:

  • Solid phase microextraction offers a streamlined solution for in vivo metabolomics.
  • This technology overcomes significant challenges in tissue sample collection and preparation.
  • The method shows promise for advancing biomarker discovery in complex biological processes like transplantation.