Related Experiment Video
Updated: May 6, 2026
A Customizable Approach for the Enzymatic Production and Purification of Diterpenoid Natural Products
Published on: October 4, 2019
[Extraction and purification of isocorydine from Dicranostigma leptopodum]
Yan Feng1, Ai-Mei Yang, Jun-Xi Liu
1College of Life Science & Engineering, Lanzhou University of Technology, Lanzhou 730050, China.
Objective:
To optimize the conditions of extraction and purification of isocorydine from Dicranostigma leptopodum.
Methods:
Extraction conditions of isocorydine were selected on the basis of orthogonal experimental design, the static adsorption/desorption experiments were used to evaluate the optimum resin.
Results:
The optimum extraction parameters were as follows: the ratio of raw materials to solvent 1:15 (g/mL), extraction solvent 1% vitriol, the extraction three times and 1 h each time. LX28 resin exhibited higher adsorption efficiency.
Conclusion:
Under the above optimum conditions, the extraction yield of isocorydine is 0.88%. The purity of isocorydine can reach 85.34% with a yield rate of 68.64%.
More Related Videos
08:20Systematic Approach to Identify Novel Antimicrobial and Antibiofilm Molecules from Plants' Extracts and Fractions to Prevent Dental Caries
Published on: March 31, 2021
08:56Transient Expression in Nicotiana Benthamiana Leaves for Triterpene Production at a Preparative Scale
Published on: August 16, 2018