Related Experiment Video
Updated: May 5, 2026

Synthesis and Mass Spectrometry Analysis of Oligo-peptoids
Published on: February 21, 2018
Surface-induced dissociation of multiply protonated peptides
A L McCormack1, J L Jones, V H Wysocki
1Department of Chemistry, Virginia Commonwealth University, 23284-2006, Richmond, VA.
Abstract:
We report here surface-induced dissociation spectra of three multiply charged peptides: doubly protonated angiotensin I, doubly protonated renin substrate, and triply protonated melittin. For comparison, the collision-activated dissociation spectra of renin substrate and melittin are also presented. The spectra show that surface-induced dissociation provides structural information on multiply charged peptides at the picomole per microliter sample concentrations compatible with electrospray ionization. For multiply protonated angiotensin I, renin substrate, and melittin, surface collisions (l00-165 eV) favor a limited number of fragmentation pathways, which are the same as those favored in collision-activated dissociation experiments.
Related Concept Videos
Tandem Mass Spectrometry
Peptide Identification Using Tandem Mass Spectrometry
This technique helps gather information regarding the protein from which the peptide was obtained and to study the peptides’ amino acid sequence. Identifying peptides from a complex mixture is an important component of the growing field of...
Mass Spectrometry: Carboxylic Acid, Ester, and Amide Fragmentation
For example,...
Mass Spectrometry: Molecular Fragmentation Overview
One type of fragmentation pattern is the cleavage of a single bond in the molecular ion. The cleavage leads to a radical and a cation. The cleavage can occur at...
¹³C NMR: Distortionless Enhancement by Polarization Transfer (DEPT)
Mass Spectrometry of Amines

