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Multisample probe for fast-atom bombardment / mass spectrometry.

M M Siegel1, R Tsao, W Bouley

  • 1American Cyanamid Company, Medical Research Divsion, Trderlr Laboratories, 10965, Pearl River, NY, USA.

Journal of the American Society for Mass Spectrometry
|November 16, 2013
PubMed
Summary
This summary is machine-generated.

A new, inexpensive multisample fast-atom bombardment (FAB) probe assembly was developed for the VG ZAB-SE mass spectrometer. This high-throughput system doubles sample analysis speed and sensitivity without cross-contamination.

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Area of Science:

  • Analytical Chemistry
  • Mass Spectrometry Instrumentation

Background:

  • Fast-atom bombardment (FAB) is a key ionization technique in mass spectrometry.
  • High-throughput analysis requires efficient sample introduction and handling.

Purpose of the Study:

  • To design and implement an inexpensive, high-throughput multisample probe assembly for FAB mass spectrometry.
  • To improve sample analysis efficiency and sensitivity on a VG ZAB-SE mass spectrometer.

Main Methods:

  • Development of a vacuum lock system integrated with a multisample FAB probe.
  • The probe features a tip with multiple sample wells (≥5) for sequential analysis.
  • Integration into the VG ZAB-SE mass spectrometer source region with perpendicular beam alignment.

Main Results:

  • Achieved double the sample throughput compared to a coaxial probe.
  • Demonstrated approximately double the sensitivity.
  • Confirmed no sample cross-contamination between wells.

Conclusions:

  • The designed multisample FAB probe assembly offers a cost-effective solution for high-throughput analysis.
  • The system significantly enhances analytical efficiency and sensitivity in mass spectrometry.
  • This innovation is valuable for laboratories requiring rapid and reliable sample analysis.