Effect of cinnamoyl putrescines on in vitro cell multiplication and differentiation of tobacco explants
C Martin1, G Kunesch, J Martin-Tanguy
1Station de Physiopathologie, INRA, BV 1540, F-21034, Dijon Cédex, France.
Plant Cell Reports
|November 21, 2013
Abstract:
Hydroxycinnamoyl putrescines promote the cell multiplication of leaf discs of a tobacco mutant, RMB7, cultivatedin vitro on the Murashige and Skoog medium. This mutant never accumulates these molecules during its development and does not enter in floweirng. Maximal effect is obtained at 2.5·10(-4)M. The same molecules inhibit bud formation ofNicotiana tabacum var. Xanthi nc, at 5·10(-5) M but promote callus formation. From 10(-4) M to 5·10(-3) M they strongly inhibit cell multiplication and bud formation without toxic effect. Their possible role in plant metabolism is discussed.
Related Concept Videos
02:57Plant Tissue Culture
33.4K
Plant tissue culture is widely used in both primary and applied science. Applications range from plant development studies to functional gene studies, crop improvement, commercial micropropagation, virus elimination, and conservation of rare species.
33.4K
01:21Cell Culture
17.1K
Most vertebrate cells grow in vitro attached to a substrate as a monolayer, called adherent cultures. The flasks and plates used to grow cells are chemically treated to facilitate cell attachment. However, a few cell types, such as hematopoietic cells, can grow in a suspension. In contrast to adherent cultures, suspension cultures can grow in non-treated cultureware using magnetic stirrers or spinner flasks to agitate the culture media
17.1K