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Published on: August 14, 2019
Analysis of flavonoids and iridoids in Vitex negundo by HPLC-PDA and method validation
Somendu K Roy1, Khemraj Bairwa, Jagdeep Grover
1Department of Natural Products, National Institute of Pharmaceutical Education and Research (NIPER), Sector- 67, SAS Nagar, Mohali- 160062, Punjab, India.
Abstract:
The leaves of Vitex negundo have been reported to contain various bioactive constituents including iridoids and flavonoids. This is the first report on the simultaneous determination of iridoids and flavonoids by HPLC in three different samples of V. negundo leaves collected from three regions of India. Separation of iridoids and flavonoids was accomplished by HPLC and further elaborated for their quantification in V. negundo leaves using a C-18 column with detection at 254 and 330 nm, respectively. The developed HPLC method showed good linearity (r2 > or = 0.999), high precision (RSD < 5%) and a good recovery (99.3-103.0%) of the compounds. All the validation parameters of the developed HPLC were found to be within the permissible limits according to the ICH guidelines. The developed method was robust, accurate and reliable for the quality control of V. negundo leaves.
