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Updated: May 5, 2026
Comprehensive DNA Methylation Analysis Using a Methyl-CpG-binding Domain Capture-based Method in Chronic Lymphocytic Leukemia Patients
Published on: June 16, 2017
Triplex-mediated analysis of cytosine methylation at CpA sites in DNA
Marie W Johannsen1, Simon R Gerrard, Tracy Melvin
1School of Chemistry, University of Southampton, Highfield, Southampton SO17 1BJ, UK. tom.brown@chem.ox.ac.uk.
Abstract:
Modified triplex-forming oligonucleotides distinguish 5-methyl cytosine from unmethylated cytosine in DNA duplexes by differences in triplex melting temperatures. The discrimination is sequence-specific; dramatic differences in stabilisation are seen for CpA methylation, whereas CpG methylation is not detected. This direct detection of DNA methylation constitutes a new approach for epigenetic analysis.
