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Updated: May 5, 2026

Navigating the Mass Spectrometry-Based Proteomic Data Using Free Computational Tools
Published on: August 19, 2025
DeNovoGUI: an open source graphical user interface for de novo sequencing of tandem mass spectra
Thilo Muth1, Lisa Weilnböck, Erdmann Rapp
1Max Planck Institute for Dynamics of Complex Technical Systems , Sandtorstraße 1, 39106 Magdeburg, Germany.
Abstract:
De novo sequencing is a popular technique in proteomics for identifying peptides from tandem mass spectra without having to rely on a protein sequence database. Despite the strong potential of de novo sequencing algorithms, their adoption threshold remains quite high. We here present a user-friendly and lightweight graphical user interface called DeNovoGUI for running parallelized versions of the freely available de novo sequencing software PepNovo+, greatly simplifying the use of de novo sequencing in proteomics. Our platform-independent software is freely available under the permissible Apache2 open source license. Source code, binaries, and additional documentation are available at http://denovogui.googlecode.com .
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