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Using Mouse Oocytes to Assess Human Gene Function During Meiosis I
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Method of cytogenetic assay of mouse oocytes
K L Pligina1, A K Zhanataev, Z V Chaika
1V. V. Zakusov Research Institute of Pharmacology, Russian Academy of Medical Sciences, Moscow, Russia. kira-pligina@rambler.ru.
Bulletin of Experimental Biology and Medicine
|December 10, 2013
Abstract:
We developed an original method of isolation and analysis of cytogenetic micropreparations of mouse oocytes including treatment with buffered hypotonic saline, paraformaldehyde fixation, and fluorescent staining. The method had several advantages, including high quality of sections and low labour intensity.
