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Updated: May 4, 2026

Multi-analyte Biochip MAB Based on All-solid-state Ion-selective Electrodes ASSISE for Physiological Research
Published on: April 18, 2013
Development of stable and reproducible biosensors based on electrochemical impedance spectroscopy: three-electrode
Luca Ianeselli1, Gianluca Grenci2, Carlo Callegari3
1SISSA, via Bonomea, 265, 34136 Trieste, Italy.
Abstract:
This work focuses on the development of electrochemical impedance biosensors based on capacitance readout, for the detection of biomolecules in small sample volumes. We performed electrochemical impedance spectroscopy (EIS) measurements of DNA hybridization in electrochemical cells with microfabricated gold electrodes. The time stability of the device was tested in two different configurations: two microelectrodes in a microfluidic channel; two microelectrodes plus a reference electrode in an electrochemical cell. Our results demonstrate that the three-electrode setup is more stable, more reproducible, and suitable for real-time measurements. In the last part of the work we perform a test study of DNA hybridization in real time, and we show that the three-electrode configuration can measure the process in situ and in real time.
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