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Related Experiment Videos

Nucleolar organizer regions in lymphomas.

J Crocker, P Nar

    The Journal of Pathology
    |February 1, 1987
    PubMed
    Summary

    Silver staining of nucleolar organizer region-associated proteins (Ag-NORs) effectively differentiates lymphoma grades. Higher Ag-NOR counts indicate high-grade lymphomas, aiding tumor histopathology.

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    Expression of the cellular FLICE-inhibitory protein (c-FLIP) protects Hodgkin's lymphoma cells from autonomous Fas-mediated death.

    Proceedings of the National Academy of Sciences of the United States of America·2004

    Area of Science:

    • Histopathology
    • Oncology
    • Cytogenetics

    Background:

    • Nucleolar organizer regions (NORs) and their associated proteins (Ag-NORs) are involved in cell proliferation.
    • Ag-NOR staining is a technique primarily used in cytogenetics.
    • Tumor histopathology often requires methods to assess cell proliferation and grade.

    Purpose of the Study:

    • To investigate the utility of silver staining for nucleolar organizer region-associated proteins (Ag-NORs) in paraffin-embedded non-Hodgkin's lymphoma samples.
    • To determine if Ag-NOR counts can differentiate between low-grade and high-grade lymphomas.
    • To assess the applicability of Ag-NOR staining in routine tumor histopathology.

    Main Methods:

    • Paraffin-embedded sections of 90 non-Hodgkin's lymphomas, 5 palatine tonsils, and 5 reactive lymph nodes were analyzed.
    • A silver staining technique was employed to visualize nucleolar organizer region-associated proteins (Ag-NORs).
    • Ag-NORs within the nuclei were enumerated using microscopy.

    Main Results:

    • The silver staining technique was readily applicable to paraffin sections, allowing for easy enumeration of Ag-NORs.
    • A significant difference in the mean number of Ag-NORs per nucleus was observed between low-grade lymphomas (1-1.5) and high-grade lymphomas (4.4-6.8).
    • Ag-NOR regions were detectable even in nuclei where nucleoli were not visibly apparent.

    Conclusions:

    • Ag-NOR staining is a valuable and applicable method for tumor histopathology, particularly in the assessment of non-Hodgkin's lymphomas.
    • The number of Ag-NORs can serve as a reliable indicator to distinguish between low-grade and high-grade lymphomas.
    • This technique offers a potential tool for improving the diagnostic accuracy and grading of lymphomas.

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