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[Application of PulseNet China Database in tracking and warning 4 cholerae outbreaks in Anhui province, in 2012]

Shou-kui Hu1, Tian-li Zheng2, Wan-fu Hu1

  • 1Anhui Center for Disease Control and Prevention, Hefei 230061, China.

Zhonghua Liu Xing Bing Xue Za Zhi = Zhonghua Liuxingbingxue Zazhi
|January 1, 2014
PubMed

Insights

Four cholera outbreaks in Anhui province in 2012 were linked to strains from Hunan and Sichuan. The PulseNet China Database (PNCD) effectively tracked the infection source, aiding early outbreak warnings.

Area of Science:

  • Microbiology
  • Epidemiology
  • Genetics

Background:

  • Cholera outbreaks pose significant public health challenges.
  • Accurate source tracking is crucial for effective outbreak control.

Purpose of the Study:

  • To investigate the origins of four Vibrio cholerae outbreaks in Anhui province in 2012.
  • To evaluate the utility of the PulseNet China Database (PNCD) for cholera outbreak investigations.

Main Methods:

  • Vibrio cholerae virulence genes (CT and TCP) were amplified using Polymerase Chain Reaction (PCR).
  • Bacterial strains were typed using Pulse Field Gel Electrophoresis (PFGE).
  • PFGE results were analyzed using BioNumericsV4.0 software and compared against the PNCD.

Main Results:

  • All tested Vibrio cholerae strains were positive for virulence genes CT and TCP.
  • PFGE analysis revealed over 98% homology among the four Anhui outbreak strains.
  • These strains showed 100% homology with previously identified epidemic strains in Hunan and Sichuan via PNCD.

Conclusions:

  • The 2012 Anhui cholera outbreaks were strongly correlated with concurrent outbreaks in Hunan and Sichuan.
  • The PulseNet China Database (PNCD) proved effective for rapid cholera infection source tracking.
  • PNCD facilitates early warning systems for public health interventions.
Abstract

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