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Related Experiment Videos

Oncogenes and phosphatidylinositol turnover.

L C Cantley, M Whitman, S Chahwala

    Annals of the New York Academy of Sciences
    |January 1, 1986
    PubMed
    Summary

    Viral oncogenes impact cell growth by altering phosphatidylinositol (PI) turnover. A specific PI kinase associates with polyoma middle T oncoprotein, crucial for cell transformation.

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    Area of Science:

    • Cell Biology
    • Virology
    • Biochemistry

    Background:

    • Phosphatidylinositol (PI) turnover products regulate cell growth and differentiation.
    • Viral oncogenes and transforming viruses alter PI turnover pathway intermediates.
    • Enzymatic activities in the PI turnover pathway are found in immunoprecipitates of viral transforming proteins.

    Purpose of the Study:

    • To investigate the role of PI turnover in viral transformation.
    • To identify the specific PI turnover enzyme activity associated with polyoma middle T oncoprotein.
    • To determine the significance of this association for cell transformation.

    Main Methods:

    • Cell culture and viral infection/transfection.
    • Immunoprecipitation of viral oncoproteins.

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  • Enzyme activity assays (PI phosphorylation).

    • Main Results:

    • Polyoma middle T immunoprecipitates catalyze the phosphorylation of PI to phosphatidylinositol-4-phosphate (PIP).
    • This PI kinase activity is due to an associated cellular enzyme, not intrinsic to middle T or c-src.
    • The PI kinase associates with transformation-competent middle T but not with transformation-defective mutants.

    Conclusions:

    • A specific PI kinase associates with polyoma middle T oncoprotein.
    • This association is dependent on the transformation capability of the middle T protein.
    • The association of PI kinase with middle T may be a critical mechanism for viral-induced cell transformation.