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Updated: May 4, 2026
A Customizable Approach for the Enzymatic Production and Purification of Diterpenoid Natural Products
Published on: October 4, 2019
[Study on the extraction process and macroporous resin for purification of Timosaponin B II]
Yan-Ping Liu1, Yue Ding2, Tong Zhang2
1Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China. lyp1066@hotmail.com
Objective:
To optimize the extraction process and macroporous resin for purification of Timosaponin B II from Anemarrhena asphodeloides.
Methods:
Orthogonal design L9 (34) was employed to optimize the circumfluence extraction conditions by taking the extraction yield of Timosaponin B II as index. The absorption-desorption characteristics of eight kinds of macroporous resins were evaluated, then the best resin was chosen to optimize the purification process conditions.
Results:
The optimum extraction conditions were as follows: the herb was extracted for 2 times (2 hours each time) with 8.5-fold 50% ethanol at the first time and 6-fold 50% ethanol at the second time. HPD100 resin showed a good property for the absorption-desorption of Timosaponin B II. The optimum technological conditions of HPD100 resin were as follows:the solution concentration was 0.23 mg/mL, the amount of saturated adsorption at 4/5 body volumn (BV) resin, the HPD100 resin was washed with 3 BV water and 6 BV 20% ethanol solution to remove the impurity, then the Timosaponin B II was desorbed by 5 BV ethanol solution. The purity of Timosaponin B II was about 50%.
Conclusion:
The optimized extraction process and purification is stable, efficient and suitable for industrial production.
