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Updated: Jul 5, 2026

Fluorescent Silver Staining of Proteins in Polyacrylamide Gels
Published on: April 21, 2019
An excimer-based, turn-on fluorescent sensor for the selective detection of diphosphorylated proteins in aqueous
Dziyana Kraskouskaya1, Matthew Bancerz, Harjeet S Soor
1Department of Chemistry and Department of Chemical and Physical Sciences, University of Toronto, Mississauga , 3359 Mississauga Road North, Mississauga, Ontario, Canada L5L1C6.
Abstract:
Protein phosphorylation is a ubiquitous post-translational modification, which often acts as a switch to proteins' activation and is frequently perturbed in diseases. Although many general phospho-protein detection tools are available, none of them offers information about the relative spatial arrangement of phosphorylated residues. Specifically, proximally phosphorylated residues are hallmarks of certain activated disease-relevant proteins. We herein report the first turn-on fluorescent sensor for the selective detection of proximally phosphorylated protein sites, suitable for application in both aqueous solutions and polyacrylamide gels.
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