Effects of calcium entry blockers not connected with calcium channels inhibition

We tested the effects of calcium entry blockers (verapamil, nifedipine, diltiazem) in comparison with nitroglycerin to investigate their inhibitory mechanisms of smooth muscle contraction not connected with the calcium blockade through calcium channels. The experiments were carried out by using isolated rabbit aorta strips in both normal and Ca2+-free solutions. Our results indicate that nitroglycerin and calcium entry blockers act on different calcium pools; the differences observed among calcium entry blockers suggest that other factors, different from calcium channels blockade (alpha-adrenergic block, unspecific effects), may be responsible for their inhibitory effect when high doses are employed.