Bienzymatic biosensor for rapid detection of aspartame by flow injection analysis

Area of Science:

• Biotechnology
• Biosensor Technology
• Analytical Chemistry

Background:

• Aspartame detection is crucial for food quality control.
• Existing methods can be time-consuming or require complex sample preparation.
• Development of rapid, sensitive, and stable biosensors is needed.

Purpose of the Study:

• To develop a rapid, simple, and stable biosensor for aspartame detection.
• To immobilize key enzymes and modify electrode surfaces for enhanced performance.
• To evaluate the biosensor's efficiency in terms of response time, throughput, and detection limits.

Main Methods:

• Immobilization of alcohol oxidase (AOX), carboxyl esterase (CaE), and bovine serum albumin (BSA) using glutaraldehyde (GA).
• Modification of screen-printed electrodes with cobalt-phthalocyanine (CoPC).
• Utilizing a flow injection analysis (FIA) system for high-throughput measurements.

Main Results:

• The developed biosensor demonstrated a fast response time.
• High sample throughput of 40 samples per hour with a relative standard deviation (RSD) of 2.7% was achieved using FIA.
• Detection limits were as low as 0.1 µM for methanol and 0.2 µM for aspartame.
• Successful application in determining aspartame in various liquid and solid food samples without pre-treatment.

Conclusions:

• The developed enzymatic biosensor is rapid, simple, and stable for aspartame detection.
• The biosensor shows excellent performance metrics, including high throughput and low detection limits.
• It is a viable tool for aspartame determination in diverse food matrices, simplifying quality control processes.