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Transmission Electron Microscopy

Transmission Electron Microscopy

In 1931, physicist Ernst Ruska—building on the idea that magnetic fields can direct an electron beam just as lenses can direct a beam of light in an optical microscope—developed the first prototype of the electron microscope. This development led to the development of the field of electron microscopy. In the transmission electron microscope (TEM), electrons are produced by a hot tungsten element and accelerated by a potential difference in an electron gun, which gives them up to 400...

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Thermal Measurement Techniques in Analytical Microfluidic Devices

Thermal Measurement Techniques in Analytical Microfluidic Devices

Published on: June 3, 2015

Thermal lens microscopy as a detector in microdevices.

Christopher L Cassano¹, Kazuma Mawatari, Takehiko Kitamori

¹Department of Mechanical and Aerospace Engineering, University of Florida, Gainesville, FL, USA.

Electrophoresis

|January 18, 2014

Summary

Thermal Lens Microscopy (TLM) offers a sensitive, flexible alternative to fluorescence detection for microfluidic applications. This photothermal technique overcomes limitations like weak signals and photobleaching, enabling precise quantification.

Keywords:

Detection Microfluidics Thermal lens microscopy

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Thermal Measurement Techniques in Analytical Microfluidic Devices

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Area of Science:

Analytical Chemistry
Spectroscopy
Microfluidics

Background:

Microfluidic devices require highly sensitive detection methods for low concentrations in small volumes.
Fluorescence detection is common but faces challenges like weak signals, autofluorescence, and photobleaching.
Existing detection methods in microfluidics have limitations that necessitate alternative approaches.

Purpose of the Study:

To introduce and advocate for Thermal Lens Microscopy (TLM) as a superior detection method for microfluidics.
To highlight TLM's advantages over traditional fluorescence detection in microfluidic systems.
To encourage wider adoption of TLM in microfluidic applications, including microchip-based capillary electrophoresis (CE).

Main Methods:

Review of photothermal spectroscopy techniques, specifically focusing on Thermal Lens Microscopy (TLM).
Comparison of TLM's capabilities with established methods like fluorescence detection in microfluidic contexts.
Discussion of TLM's application in sensitive quantification and label-free detection.

Main Results:

TLM is a sensitive and flexible detection approach for nonfluorescent molecules.
TLM effectively overcomes common limitations associated with fluorescence detection.
TLM facilitates single-molecule detection and label-free in vivo quantification within microfluidic systems.

Conclusions:

Thermal Lens Microscopy (TLM) presents a viable and advantageous alternative to fluorescence detection in microfluidics.
Wider implementation of TLM can enhance the capabilities of microchip-based CE and other microfluidic technologies.
TLM's unique properties make it a promising tool for advancing microfluidic analysis.