Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Evaluation of <i>in vitro</i> blood-brain barrier models in central nervous system new drug development: methodological challenges and translational perspectives.

Expert opinion on drug delivery·2026
Same author

VAPB confers selective neuroprotection by driving autophagic degradation of pathogenic aggregates in ALS.

Acta neuropathologica communications·2026
Same author

The Role of Indocyanine Green Videoangiography in Intramedullary Spinal Cord Tumor Surgery: Focus on Lesion Localization Accuracy.

Neurologia medico-chirurgica·2026
Same author

Spatiotemporal Dynamics in Prespeech Semantic Category Decoding: An Intracranial EEG Study.

eNeuro·2026
Same author

Long-term radiological progression after resection of dysembryoplastic neuroepithelial tumors: patterns and prognostic factors.

Journal of neuro-oncology·2026
Same author

Figure-ground relationship of voices in musical structure modulates reciprocal frontotemporal connectivity.

Frontiers in neuroscience·2026
Same journal

Opioid-Associated Hippocampal Injury: Past, Present, and Future Directions.

Hippocampus·2026
Same journal

Neural and Navigational Features Influencing the Novelty Induced Benefits on Episodic Memory.

Hippocampus·2026
Same journal

Intrinsic Persistent Firing in CA1 Encodes Elapsed Time Across Behaviorally Relevant Scales.

Hippocampus·2026
Same journal

Boundary Vector Cells Encode a Future-Biased Spectrum of Positions in the Rat.

Hippocampus·2026
Same journal

Hippocampal NOP Receptor Activation Impairs Object Recognition Memory Acquisition.

Hippocampus·2026
Same journal

Effects of Corticotropin-Releasing Factor 1 Receptor Antagonism on In Vivo Dentate Gyrus Long-Term Potentiation in the TgF344-AD Rat Model of Alzheimer's Disease.

Hippocampus·2026
See all related articles

Related Experiment Video

Updated: May 3, 2026

Multi-electrode Array Recordings of Human Epileptic Postoperative Cortical Tissue
13:14

Multi-electrode Array Recordings of Human Epileptic Postoperative Cortical Tissue

Published on: October 26, 2014

23.4K

Human temporal lobe epilepsy analyses by tissue proteomics.

Céline Mériaux1, Julien Franck, Dan Bi Park

  • 1Laboratoire de Spectrométrie de Masse Biologique Fondamentale et Appliquée-EA 4550, Bât SN3, 1er étage, Université de Lille 1, Villeneuve d'Ascq, France; EURON-European Graduate School of Neuroscience, Maastricht University, Maastricht, The Netherlands.

Hippocampus
|January 23, 2014
PubMed
Summary
This summary is machine-generated.

Temporal lobe epilepsy (TLE) involves complex molecular changes, including specific neuropeptides and tumor suppressors like LGI1. Gender influences these molecular differences, suggesting distinct pathology development mechanisms in TLE patients.

Keywords:
epilepsymass spectrometry imagingneuropeptideneuroproteomicsexome

More Related Videos

Network Analysis of Foramen Ovale Electrode Recordings in Drug-resistant Temporal Lobe Epilepsy Patients
09:32

Network Analysis of Foramen Ovale Electrode Recordings in Drug-resistant Temporal Lobe Epilepsy Patients

Published on: December 18, 2016

13.7K
High Resolution Quantitative Synaptic Proteome Profiling of Mouse Brain Regions After Auditory Discrimination Learning
10:36

High Resolution Quantitative Synaptic Proteome Profiling of Mouse Brain Regions After Auditory Discrimination Learning

Published on: December 15, 2016

12.2K

Related Experiment Videos

Last Updated: May 3, 2026

Multi-electrode Array Recordings of Human Epileptic Postoperative Cortical Tissue
13:14

Multi-electrode Array Recordings of Human Epileptic Postoperative Cortical Tissue

Published on: October 26, 2014

23.4K
Network Analysis of Foramen Ovale Electrode Recordings in Drug-resistant Temporal Lobe Epilepsy Patients
09:32

Network Analysis of Foramen Ovale Electrode Recordings in Drug-resistant Temporal Lobe Epilepsy Patients

Published on: December 18, 2016

13.7K
High Resolution Quantitative Synaptic Proteome Profiling of Mouse Brain Regions After Auditory Discrimination Learning
10:36

High Resolution Quantitative Synaptic Proteome Profiling of Mouse Brain Regions After Auditory Discrimination Learning

Published on: December 15, 2016

12.2K

Area of Science:

  • Neuroscience
  • Molecular Biology
  • Biochemistry

Background:

  • Temporal lobe epilepsy (TLE) is the most common and challenging form of epilepsy to treat.
  • The underlying epileptogenic process in TLE remains poorly understood despite extensive research.
  • A single cause is unlikely; multiple factors likely contribute to TLE's neuropathology.

Purpose of the Study:

  • To investigate the molecular profiles of TLE using MALDI mass spectrometry imaging (MSI).
  • To identify specific neuropeptides, protein precursors, receptors, and other molecules associated with TLE.
  • To explore the influence of gender on molecular changes in TLE.

Main Methods:

  • Tissue microextraction from 10 TLE patients and 10 controls.
  • Trypsin digestion followed by nanoLC separation coupled to LTQ Orbitrap.
  • Shot-gun proteomic analysis and MALDI-MSI for molecular profiling.

Main Results:

  • Identified seven neuropeptides in the hippocampus dentate gyrus of TLE patients.
  • Confirmed presence of neuropeptide precursors/receptors and proteins in axon regeneration (neurotrophins, ECM proteins).
  • Discovered a complete profile of seven tumor suppressors, including Leucine-rich glioma inactivated 1 (LGI1), and identified gender-specific protein differences.

Conclusions:

  • Molecular profiling reveals complex changes in TLE, involving neuropeptides, axon regeneration proteins, and tumor suppressors.
  • LGI1 protein, implicated in TLE heritability, was identified, along with a full set of tumor suppressors.
  • Gender-specific molecular differences suggest distinct mechanisms in TLE pathology development.