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Related Experiment Video

Updated: May 3, 2026

Semi-Automated Phenotypic Analysis of Functional 3D Spheroid Cell Cultures
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Semi-Automated Phenotypic Analysis of Functional 3D Spheroid Cell Cultures

Published on: August 18, 2023

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Three-dimensional cell culture technique and pathophysiology.

Michiya Matsusaki1, Charles Patrick Case2, Mitsuru Akashi1

  • 1Department of Applied Chemistry, Graduate School of Engineering, Osaka University, 2-1 Yamadaoka, Suita, Osaka 565-0871, Japan.

Advanced Drug Delivery Reviews
|January 28, 2014
PubMed
Summary
This summary is machine-generated.

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Three-dimensional (3D) human tissue models offer advanced applications in drug testing and disease research, replacing animal experiments. Novel fabrication technologies enable complex 3D tissue construction for precise physiological studies.

Area of Science:

  • Biomedical Engineering
  • Tissue Engineering
  • Regenerative Medicine

Background:

  • Three-dimensional (3D) human tissue constructs are valuable for pharmaceutical, pathophysiological, and tissue engineering applications.
  • Current limitations exist in precisely reconstructing multi-cellular 3D constructs due to their heterogeneous aggregation.
  • In vivo animal experiments can be supplemented by advanced 3D-human tissue models.

Purpose of the Study:

  • To provide an overview of current 3D tissue fabrication technologies.
  • To highlight the biomedical applications of these advanced 3D-human tissue models.
  • To emphasize the potential of 3D-human tissue models in understanding complex biological processes.

Main Methods:

  • Review of novel technologies for fabricating complex 3D-human tissues, including capillary networks.
Keywords:
3D constructsExtracellular matrixHuman-tissue modelLayer-by-layer assemblyNano-filmsVasculature

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  • Analysis of methods enabling nano/micro-meter scale replication of physiological human tissue responses.
  • Exploration of fabrication techniques for precise 3D cell positioning.

    • Main Results:

    • Emerging technologies allow for the development of complex 3D-human tissues with intricate structures like capillary networks.
    • These advanced models can replicate physiological human tissue responses at the nano/micro-meter scale.
    • The precise 3D-location of multiple cell types is becoming achievable, overcoming limitations of simple spheroids.

    Conclusions:

    • 3D-human tissue models represent a powerful advancement over traditional methods for various biomedical applications.
    • These models hold significant potential for estimating drug effects, studying cancer metastasis, and assessing nanomaterial toxicity.
    • Future research in 3D tissue fabrication will further enhance their utility in pathophysiological studies.