Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Protein Dynamics in Living Cells01:19

Protein Dynamics in Living Cells

1.9K
Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...
1.9K
Distribution of Molecular Speeds01:27

Distribution of Molecular Speeds

4.0K
The motion of molecules in a gas is random in magnitude and direction for individual molecules, but a gas of many molecules has a predictable distribution of molecular speeds. This predictable distribution of molecular speeds is known as the Maxwell-Boltzmann distribution. The distribution of molecular speeds in liquids is comparable to that of gases but not identical and can help to understand the phenomenon of the boiling and vapor pressure of a liquid. Consider that a molecule requires a...
4.0K
¹H NMR of Conformationally Flexible Molecules: Temporal Resolution00:52

¹H NMR of Conformationally Flexible Molecules: Temporal Resolution

992
At room temperature, the chair conformer of cyclohexane undergoes rapid ring flipping between two equivalent chair conformers at a rate of approximately 105 times per second. These two chair conformers are in equilibrium. The rapid ring flipping results in the interconversion of the axial proton to an equatorial proton and an equatorial to the axial proton. Such interconversions are too rapid and cannot be detected on the NMR timescale. Hence, the NMR spectrometer cannot distinguish between the...
992

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Restoration of Saccadic Eye Movements and Visually Guided Behavior in Ambient White Light with Photoswitchable Small Molecules.

Journal of the American Chemical Society·2026
Same author

Weather-Resistant Thermoresponsive UV-Curable Smart Window Composites Based on Paraffin Particles.

ACS applied materials & interfaces·2026
Same author

Coupling Single Molecules to DNA-Based Optical Antennas with Position and Orientation Control.

ACS photonics·2026
Same author

Leveraging Pretrained Neural Network Models for the Classification of Tumor Cells Analyzed by Label-Free Phase Holotomographic Microscopy.

Computational and structural biotechnology journal·2026
Same author

Solvent Effects on the Blinking Statistics of Color Centers in Chemical Vapor Deposition-Grown hBN.

ACS omega·2026
Same author

Distinct nanoscale membrane organizations of mucins and <i>trans</i>-sialidases in <i>Trypanosoma cruzi</i>.

bioRxiv : the preprint server for biology·2026

Related Experiment Video

Updated: May 3, 2026

An Analog Macroscopic Technique for Studying Molecular Hydrodynamic Processes in Dense Gases and Liquids
11:03

An Analog Macroscopic Technique for Studying Molecular Hydrodynamic Processes in Dense Gases and Liquids

Published on: December 4, 2017

7.6K

Ultrafast dynamics of single molecules.

Daan Brinks1, Richard Hildner, Erik M H P van Dijk

  • 1ICFO - Institut de Ciencies Fotoniques, Mediterranean Technology Park, 08860 Castelldefels, Barcelona, Spain. Niek.vanHulst@ICFO.eu.

Chemical Society Reviews
|January 30, 2014
PubMed
Summary

Femtosecond single molecule spectroscopy reveals ultrafast electronic and vibrational dynamics. This advanced technique enables quantum control and investigation of photosynthetic light-harvesting complexes.

More Related Videos

Study of Protein Dynamics via Neutron Spin Echo Spectroscopy
08:03

Study of Protein Dynamics via Neutron Spin Echo Spectroscopy

Published on: April 13, 2022

1.8K
Measurement of Ultrafast Vibrational Coherences in Polyatomic Radical Cations with Strong-Field Adiabatic Ionization
08:22

Measurement of Ultrafast Vibrational Coherences in Polyatomic Radical Cations with Strong-Field Adiabatic Ionization

Published on: August 6, 2018

6.5K

Related Experiment Videos

Last Updated: May 3, 2026

An Analog Macroscopic Technique for Studying Molecular Hydrodynamic Processes in Dense Gases and Liquids
11:03

An Analog Macroscopic Technique for Studying Molecular Hydrodynamic Processes in Dense Gases and Liquids

Published on: December 4, 2017

7.6K
Study of Protein Dynamics via Neutron Spin Echo Spectroscopy
08:03

Study of Protein Dynamics via Neutron Spin Echo Spectroscopy

Published on: April 13, 2022

1.8K
Measurement of Ultrafast Vibrational Coherences in Polyatomic Radical Cations with Strong-Field Adiabatic Ionization
08:22

Measurement of Ultrafast Vibrational Coherences in Polyatomic Radical Cations with Strong-Field Adiabatic Ionization

Published on: August 6, 2018

6.5K

Area of Science:

  • Physical Chemistry
  • Quantum Optics
  • Molecular Biophysics

Background:

  • Single-molecule detection is crucial in various scientific fields, enabling detailed studies of molecular processes.
  • Traditional methods track molecular dynamics over time, but studying ultrafast dynamics at the single-molecule level is challenging.

Purpose of the Study:

  • To review recent advancements in femtosecond single-molecule spectroscopy for studying ultrafast molecular dynamics.
  • To demonstrate the application of these techniques for observing electronic and vibrational coherence and quantum control.

Main Methods:

  • Confocal pump-probe spectroscopy.
  • Deterministic coherent control using pulse shapers and ultra-broadband laser systems.
  • Two-color phase shaping for investigating photosynthetic complexes.

Main Results:

  • Detection of electronic and vibrational femtosecond dynamics in individual fluorophores at room temperature.
  • Observation of electronic (de)coherence, vibrational wavepacket interference, and quantum control.
  • Investigation of persistent coherence in individual photosynthetic light-harvesting complexes under physiological conditions.

Conclusions:

  • Femtosecond single-molecule spectroscopy is a powerful tool for probing ultrafast molecular dynamics.
  • Coherent control techniques offer new possibilities for manipulating and understanding molecular processes.
  • This approach provides insights into the fundamental dynamics of complex biological systems like photosynthetic complexes.