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Published on: August 18, 2023
Proteomic analysis of human-derived cell culture supplements
Jasmin Kemptner1, Karin Witzeneder1, Simone Hennerbichler1
1Red Cross Blood Transfusion Service of Upper Austria ; Austrian Cluster for Tissue Regeneration, Linz, Austria.
Human-derived supplements like human serum (huS) and human platelet lysate (hPL) offer alternatives to fetal bovine serum (FBS) for cell expansion. Proteomic analysis revealed distinct protein profiles among these supplements, impacting cell therapy development.
Area of Science:
- Cell biology and regenerative medicine
- Proteomics and analytical chemistry
Background:
- Cell therapy and advanced therapy medicinal products require in vitro cell expansion.
- Fetal bovine serum (FBS) is commonly used but human-derived supplements are sought as alternatives.
Purpose of the Study:
- To compare the proteomic profiles of human serum (huS) and two forms of human platelet lysate (hPLP and hPLN).
- To evaluate these supplements as alternatives to FBS for cell culture.
Main Methods:
- Utilized 2D gel electrophoresis for proteomic analysis.
- Compared protein patterns, number, and concentration across huS, hPLP, and hPLN.
Main Results:
- Significant differences in protein profiles were observed between huS and hPL-containing supplements.
- Distinct variations in protein number and concentration were detected.
Conclusions:
- Human serum (huS) and human platelet lysate (hPL) exhibit unique proteomic signatures.
- These differences are crucial for selecting optimal supplements in cell therapy development.
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