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Updated: Aug 11, 2026
Use of Interferon-γ Enzyme-linked Immunospot Assay to Characterize Novel T-cell Epitopes of Human Papillomavirus
Published on: March 8, 2012
[Expression of HPV16 E7 protein and preparation of its polyclonal antibody]
Bingbing Wang1, Jianxin Tu, Yan Lv
1Institute of Molecular Virology and Immunology, Department of Medical Microbiology and Immunology, Wenzhou Medical University, Wenzhou 325000, China.
Objective:
To prepare a prokaryotic expression vector carrying E7 protein of human papillomavirus (HPV) type 16 and a polyclonal antibody against it.
Methods:
The HPV16 E7 gene was amplified by PCR from the tissue samples of cervical cancer and cloned into the pET21a(+) prokaryotic expression vector. The constructed pET21a(+)/HPV16 E7 recombinant plasmid was transformed into E.coli BL21(DE3) and induced by isopropyl beta-D-1-thiogalactopyranoside (IPTG). The recombinant protein of HPV16 E7 was purified by Ni-NTA affinity chromatography and confirmed by SDS-PAGE and Western blot analysis. To prepare the polyclonal antibody, the rabbits were immunized with the purified recombinant protein HPV16 E7. The titers of specific antibodies were detected by ELISA. The specificity activity was further detected by Western blotting and immunofluorescence analysis.
Results:
HPV16 E7 recombinant protein was expressed and purified in the prokaryotic expression system. The polyclonal antibodies were produced in the rabbits immunized with the recombinant protein. The titer of specific antibodies was up to 1:30 000. Western blotting and immunofluorescence analysis indicated that the polyclonal antibody could specifically recognize the HPV16 E7 protein.
Conclusion:
HPV16 E7 recombinant protein was successfully expressed and its polyclonal antibody was prepared.
