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Updated: May 3, 2026
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Evaluation of Biomaterials for Bladder Augmentation using Cystometric Analyses in Various Rodent Models
Published on: August 9, 2012
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The use of mesenchymal stem cells in bladder augmentation
Mila Torii Corrêa Leite1, Luiz G Freitas-Filho, Andréia Silva Oliveira
1Department of Surgery, Federal University of São Paulo, Rua Batista Cepelos 87-ap 61, São Paulo, 04109-120, Brazil, milatorii@gmail.com.
Pediatric Surgery International
|February 7, 2014
Summary
Mesenchymal stem cells (MSCs) improved bladder muscle repair. Intravenous MSCs enhanced nerve regeneration, showing potential for bladder tissue repair and functional recovery.
Area of Science:
- Regenerative Medicine
- Biomaterials Science
- Urology
Background:
- Bladder acellular matrix (BAM) is a promising scaffold for tissue regeneration.
- Mesenchymal stem cells (MSCs) possess regenerative and immunomodulatory properties.
- Optimizing MSC delivery methods is crucial for effective bladder augmentation.
Purpose of the Study:
- To compare the efficacy of bladder acellular matrix (BAM) seeded with mesenchymal stem cells (MSCs) versus intravenous (IV) MSC administration for bladder repair.
- To evaluate the integration and impact of MSCs on bladder tissue regeneration and neural repair.
Main Methods:
- MSCs were isolated from rat bone marrow.
- Rats underwent bladder augmentation with BAM, with or without MSCs (seeded or IV administered).
- Histological, immunohistochemical, and gene expression analyses were performed at 7, 14, and 28 days post-operation.
Main Results:
- Both BAM augmentation with IV MSCs and BAM-MSC seeded augmentation showed improved muscle layer repair.
- Intravenous MSC administration led to the best neural repair and the least inflammatory reaction by day 28.
- No MSCs were detected within the bladder graft itself in any group.
Conclusions:
- MSCs significantly enhance the repair of the bladder muscle layer.
- Intravenous administration of MSCs promotes neuronal regeneration, suggesting a potential therapeutic strategy for bladder repair.
