Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Video

Updated: May 3, 2026

Compact Quantum Dots for Single-molecule Imaging
17:14

Compact Quantum Dots for Single-molecule Imaging

Published on: October 9, 2012

17.5K

Fluorescent nanodiamonds embedded in biocompatible translucent shells.

Ivan Rehor1, Jitka Slegerova, Jan Kucka

  • 1Institute of Organic Chemistry and Biochemistry AS CR, v.v.i. Flemingovo nam. 2, Prague 6, 166 10, Czech Republic.

Small (Weinheim an Der Bergstrasse, Germany)
|February 7, 2014
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Streamlined molecular farming of plant virus therapeutics for space flight and other low-resource environments.

NPJ science of plants·2026
Same author

Rounded Monodisperse Fluorescent Nanodiamonds.

Small (Weinheim an der Bergstrasse, Germany)·2026
Same author

Cowpea mosaic virus as a candidate for intratumoral immunotherapy: progress, challenges, and future directions.

Advanced drug delivery reviews·2026
Same author

A strong, reversible, and conformal adhesive gel for diverse plants.

Science advances·2026
Same author

Comparative analyses for plant virus-based cancer immunotherapy drug development.

Cell biomaterials·2026
Same author

Plant-Produced Viral Nanoparticles Decorated with Nanobodies Against HER2 Improve Retention and Recruitment of Immune Cells in Solid Tumors.

Advanced healthcare materials·2026
Same journal

A Droplet-Microarray Platform for Multiplex Profiling of Breast Cancer Exosome Subtypes in Patients' Blood Plasma Samples.

Small (Weinheim an der Bergstrasse, Germany)·2026
Same journal

Material-Dependent Functionalization of CVD-Grown TMDC Monolayers Probed by Vibrational Nanospectroscopy.

Small (Weinheim an der Bergstrasse, Germany)·2026
Same journal

BandGap Modulated Charge Gating of Semiconductor Coatings Stabilizes Zinc Metal Anodes.

Small (Weinheim an der Bergstrasse, Germany)·2026
Same journal

For High Capacity: Upcycling of Spent Graphite Catalytic via Precisely Tailoring Water-Gas Reaction.

Small (Weinheim an der Bergstrasse, Germany)·2026
Same journal

Electronic Engineering of Donor-Acceptor Covalent Organic Frameworks via Fluorine Substitution for Efficient Solar Hydrogen Production.

Small (Weinheim an der Bergstrasse, Germany)·2026
Same journal

Correction to: "A Gold Nanocage/Cluster Hybrid Structure for Whole-Body Multispectral Optoacoustic Tomography Imaging, EGFR Inhibitor Delivery, and Photothermal Therapy".

Small (Weinheim an der Bergstrasse, Germany)·2026
See all related articles

Researchers developed silica-shelled fluorescent nanodiamonds (FNDs) for biological applications. These enhanced FNDs show improved stability, biocompatibility, and cellular penetration compared to bare nanodiamonds.

Area of Science:

  • Materials Science
  • Nanotechnology
  • Biomedical Engineering

Background:

  • High pressure high temperature (HPHT) nanodiamonds (NDs) are promising for nanoprobes and nanosensors.
  • Bare NDs exhibit limitations including precipitation in biological solutions, limited conjugation methods, and polydispersity.
  • These limitations hinder their direct application in biological environments.

Purpose of the Study:

  • To encapsulate fluorescent nanodiamonds (FNDs) within silica shells to improve their properties for biological applications.
  • To create monodisperse, near-spherical particles with enhanced stability and biocompatibility.
  • To functionalize these particles for targeted delivery and imaging using bio-orthogonal click chemistry.

Main Methods:

  • Encapsulation of 30-nm FNDs within 10-20-nm silica shells to create 66-nm particles.
Keywords:
biocompatibilizationfluorescent nanodiamondsnanoparticles

More Related Videos

Bio-inspired Polydopamine Surface Modification of Nanodiamonds and Its Reduction of Silver Nanoparticles
07:58

Bio-inspired Polydopamine Surface Modification of Nanodiamonds and Its Reduction of Silver Nanoparticles

Published on: November 14, 2018

8.0K
Synthesis of Core-shell Lanthanide-doped Upconversion Nanocrystals for Cellular Applications
13:51

Synthesis of Core-shell Lanthanide-doped Upconversion Nanocrystals for Cellular Applications

Published on: November 10, 2017

14.3K

Related Experiment Videos

Last Updated: May 3, 2026

Compact Quantum Dots for Single-molecule Imaging
17:14

Compact Quantum Dots for Single-molecule Imaging

Published on: October 9, 2012

17.5K
Bio-inspired Polydopamine Surface Modification of Nanodiamonds and Its Reduction of Silver Nanoparticles
07:58

Bio-inspired Polydopamine Surface Modification of Nanodiamonds and Its Reduction of Silver Nanoparticles

Published on: November 14, 2018

8.0K
Synthesis of Core-shell Lanthanide-doped Upconversion Nanocrystals for Cellular Applications
13:51

Synthesis of Core-shell Lanthanide-doped Upconversion Nanocrystals for Cellular Applications

Published on: November 10, 2017

14.3K
  • Modification of silica shells with polyethylene glycol (PEG) chains for solution stability.
  • Bio-orthogonal click chemistry to conjugate fluorescent dyes or targeting peptides to PEG chains.

    • Main Results:

    • Achieved monodisperse, near-spherical silica-encapsulated FNDs (mean diameter 66 nm).
    • PEGylation of shells provided excellent stability in ionic solutions.
    • High yield functionalization with ~2000 dye or peptide molecules per FND via click chemistry.
    • Demonstrated superior in vitro performance with human prostate cancer cells, showing no non-specific adsorption and cellular penetration.

    Conclusions:

    • Silica shell encapsulation significantly enhances the properties of FNDs for biological applications.
    • PEGylation and click chemistry enable stable, highly functionalized nanodiamond probes.
    • These engineered nanodiamonds offer improved biocompatibility and cellular uptake for potential use in diagnostics and therapeutics.