Optimization of complex fermentation media for glucose oxidase production using statistical approach

Area of Science:

• Biotechnology
• Enzyme Engineering
• Industrial Microbiology

Background:

• Enzyme production costs are heavily influenced by strain selection and raw materials.
• Aspergillus terreus UniMAP AA-1 has shown promise as a superior source for Glucose oxidase (GOx).
• Utilizing cost-effective substrates like molasses can reduce overall enzyme production expenses.

Purpose of the Study:

• To optimize media components for enhanced Glucose oxidase (GOx) production.
• To investigate the use of locally sourced Aspergillus terreus UniMAP AA-1 and sugarcane molasses for GOx synthesis.
• To establish cost-effective conditions for crude GOx enzyme production.

Main Methods:

• A locally isolated strain, Aspergillus terreus UniMAP AA-1, was used for enzyme production.
• Sugarcane molasses, supplemented with urea and carbonate salt, served as the primary growth medium.
• Response Surface Methodology (RSM) was employed for statistical optimization of media components.

Main Results:

• The highest GOx activity was achieved with specific concentrations of molasses (32.51% w/v), carbonate salt (4.58% w/v), and urea (0.93% w/v).
• Optimized conditions using readily available raw materials led to significant crude GOx enzyme activity.
• The study successfully demonstrated a cost-effective approach to GOx production.

Conclusions:

• Optimized media formulations using local resources can significantly enhance GOx production.
• This research offers a viable, low-cost alternative for industrial GOx enzyme manufacturing.
• The findings support the use of Aspergillus terreus UniMAP AA-1 and molasses for efficient enzyme synthesis.