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Silver methenamine impregnation: a useful adjunct to renal electron microscopy
1Department of Tissue Pathology, Prince Henry's Hospital.
Pathology
|October 1, 1987
Summary
Silver methenamine impregnation (SMI) aids renal electron microscopy (EM) in diagnosing kidney biopsies. This technique is valuable for assessing subtle ultrastructural changes in various glomerulonephritis subtypes.
Area of Science:
- Nephrology
- Pathology
- Electron Microscopy
Background:
- Renal electron microscopy (EM) is crucial for diagnosing kidney diseases.
- Silver methenamine impregnation (SMI) has been underutilized in renal biopsy analysis.
Purpose of the Study:
- To evaluate the utility of silver methenamine impregnation (SMI) in renal electron microscopy (EM).
- To assess the effectiveness of SMI in diagnosing various glomerulonephritis subtypes.
Main Methods:
- Tissues were fixed in glutaraldehyde/osmium and embedded in L.R. White resin or Epon.
- En-bloc staining with uranyl acetate was an optional procedure.
Main Results:
- SMI proved valuable for rapid assessment of renal ultrastructure.
- The technique aided in diagnosing complex or subtle changes in glomerulonephritis.
Conclusions:
- Silver methenamine impregnation (SMI) is a useful technique for renal biopsy diagnosis.
- SMI enhances the diagnosis of subtle and complex renal pathologies, including various glomerulonephritis types.