Embryo Transfer Catheter Contamination With Intravaginal Progesterone Preparations in a Simulated Embryo Transfer...

Area of Science:

• Reproductive biology
• Developmental biology
• Assisted reproductive technologies

Background:

• Intravaginal progesterone is commonly used to support luteal function during assisted reproductive technologies.
• Potential contamination of embryo transfer (ET) catheters with progesterone preparations during the procedure is a concern.
• The impact of such contamination on early embryo development requires thorough investigation.

Purpose of the Study:

• To investigate the direct effects of intravaginal progesterone exposure on mouse embryo development in vitro.
• To quantify the impact of progesterone contamination on blastocyst formation, cell number, and apoptosis.

Main Methods:

• A simulated ET model was used to expose culture medium to intravaginal progesterone preparations (gel and inserts).
• Mouse embryos were cultured in the exposed medium at varying dilutions and exposure times.
• Key developmental parameters including blastocyst progression, cell count, and apoptotic index were analyzed.

Main Results:

• Complete inhibition of blastocyst development occurred in undiluted progesterone-exposed medium.
• Embryo development and cell number improved with increased dilution of the exposed medium.
• Significant increases in embryo apoptosis and reduced cell numbers were observed with prolonged exposure.

Conclusions:

• Contamination of ET catheters with intravaginal progesterone preparations adversely affects mouse embryo development.
• Increased programmed cell death (apoptosis) is a likely mechanism contributing to impaired development.
• Careful handling during ET procedures is crucial to minimize progesterone exposure to embryos.