[Activity of white rat antioxidant system under chronic stress]

The aim of the research was to study the influence of long-term stress conditions on the rate of lipids' peroxidation (LPO), activity of antioxidant system and energy metabolism in brain, cardiac muscle and lymphocytes under 30-days isolation and diurnal cycle violation. Obtained results prove about the increasing of intensity of LPO in studied tissues under long-term stress conditions. According to the data, in lymphocytes, brain and cardiac muscle it was observed quantitative rising in nitric oxide (NO) concentrations. In junction with this it was changed the intensity of LPO, proved by the increase in quantities of products of this process. In contrast with this it was shown decrease in activity of enzymes of antioxidant system: Superoxiddismutase, Catalase, Glutathionreductase and Glutathionperoxidase, also we've studied activities of mitochondrial enzymes, such as, Succinatdehydrogenase, Creatine Kinase and Aconitase. It was shown that isolation and diurnal cycle disorders represent factors that induce remarkable down regulation of energy metabolism and increasing of oxidative stress pathways. Supposedly influence of stress factors could induce nonreversible processes that could stimulate various neurological, cardio-vascular and immune system pathologies.