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Updated: May 3, 2026
Purification of a High Molecular Mass Protein in Streptococcus mutans
Published on: September 14, 2019
[Identification of Streptococcus mutans in carious patients' saliva samples by MALDI-TOF mass spectrometry]
Wen Ren1, Feng Chen2, Yi-fei Zhang2
1Department of Preventive Dentistry, Peking University School and Hospital of Stomatology, Beijing 100081, China.
Objective:
To identify Streptococcus mutans (S. mutans) in carious patients' saliva using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS), and to establish a faster and more accurate method to identify S. mutans.
Methods:
In this study, a total of 90 carious patients from Department of Endodontics of Peking University School of Stomatology were recruited. All these patients' saliva was collected. After extracting the protein of the samples, they were identified by MALDI-TOF MS. The composite profile was analyzed using BioExplorer 1.0 software. The scores ≥ 25 were considered as S. mutans, whereas the scores <25 were as considered as non S. mutans. Finally, these results were compared with 16S rDNA sequencing to figure out the sensitivity and concordance rate, respectively.
Results:
The sensitivity of MALDI-TOF MS was 96.0%, and the concordance rate compared with 16S rDNA sequencing was as high as 98.7%.
Conclusion:
MALDI-TOF MS is high throughput, rapid and easy to perform in comparison to other conventional methods. It has a high sensivity and concordance rate. Thus, MALDI-TOF MS can serve as an effective tool for identification of S. mutans.
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