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Updated: May 2, 2026

iCLIP - Transcriptome-wide Mapping of Protein-RNA Interactions with Individual Nucleotide Resolution
Published on: April 30, 2011
Global protein-RNA interaction mapping at single nucleotide resolution by iCLIP-seq
Chengguo Yao1, Lingjie Weng, Yongsheng Shi
1Department of Microbiology and Molecular Genetics, School of Medicine, University of California, Irvine, CA, USA.
This study details a method to map RNA-binding protein interactions. Understanding these interactions is crucial for characterizing gene regulation by RNA-binding proteins.
Area of Science:
- Molecular Biology
- Genomics
- Gene Regulation
Background:
- Eukaryotic genomes contain numerous RNA-binding proteins (RBPs).
- RBPs are essential regulators of gene expression.
- Characterizing RBP targets is key to understanding their function.
Purpose of the Study:
- To provide a detailed protocol for mapping protein-RNA interactions.
- To functionally characterize mRNA 3' processing factors.
Main Methods:
- UV crosslinking and immunoprecipitation (CLIP).
- High-throughput sequencing (HTS).
- Detailed procedural description for CLIP-HTS.
Main Results:
- Established a robust CLIP-HTS protocol.
- Enabled detailed characterization of protein-RNA interactions for specific factors.
Conclusions:
- The described method is a standard for mapping RBP-RNA interactions.
- This protocol facilitates functional characterization of gene regulatory proteins.
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