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Related Concept Videos

Testes: Histology01:27

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A tough, fibrous membrane, the tunica albuginea, covers the testes, extending inward to form fibrous partitions or septa, dividing them into internal compartments called lobules. Each lobule has 1 to 3 tightly coiled seminiferous tubules where sperm production occurs. These tubules merge into a tubular network at the back of the testis, known as the rete testis. It connects to 15 to 20 efferent ductules, leading to the epididymis.
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The testes, also known as testicles, are the male gonads. They are housed within the scrotum, a sac-like structure located beneath the penis. The scrotum's primary role is to regulate the temperature of the testes, which is crucial for sperm production.
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Related Experiment Video

Updated: May 2, 2026

Simple and Efficient Technique for the Preparation of Testicular Cell Suspensions
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Testicular perfusion apparatus.

Kalanghot Padmanabhan Skandhan1, James Valsa, Rajesh Melta

  • 12 Department of Physiology, Sree Narayana Institute of Medical Sciences, Chalakka, Eranakulam - India.

Urologia
|February 22, 2014
PubMed
Summary

Researchers developed a new, simple glass perfusion apparatus for studying rabbit and rat testes. This reliable instrument is easily fabricated and suitable for regular laboratory use in reproductive biology research.

Area of Science:

  • Reproductive Biology
  • Physiology
  • Laboratory Instrumentation

Background:

  • Reliable instruments are crucial for reproductive biology research.
  • Existing testicular study methods lack suitable instrumentation.
  • A need exists for improved techniques in testicular research.

Purpose of the Study:

  • To design and fabricate a novel testicular perfusion apparatus.
  • To address the lack of adequate instruments for testicular studies.
  • To provide a reliable tool for reproductive biology laboratories.

Main Methods:

  • A glass perfusion apparatus was designed and fabricated for rabbit and rat testes.
  • Krebs Ringer bicarbonate buffer was used as the perfusion fluid.
  • A 26-gauge needle facilitated perfusate entry into the spermatic artery.

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Main Results:

  • The newly designed testicular perfusion apparatus was successfully fabricated using glass.
  • Initial tests with animal testes confirmed the apparatus's functional efficiency.
  • The instrument demonstrated reliable performance for laboratory use.

Conclusions:

  • A simple and effective perfusion apparatus for rabbit and rat testes has been developed.
  • The glass apparatus is preparable using standard glass blowing facilities.
  • This instrument offers a practical solution for routine laboratory applications in testicular research.