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Updated: Mar 14, 2026

Flow Cytometric Analysis of Biomarkers for Detecting Human Sperm Functional Defects
Published on: April 21, 2022
Level of copper in human split ejaculate
Kalanghot Skandhan1,2, James Valsa2, Balakrishnan Sumangala1,3
1Department of Physiology, Government Medical College, Surat, Gujarat - India.
Purpose:
The purpose of this study was to understand the details of splits of an ejaculate and to locate the origin of release of copper into semen.
Methods:
Laboratory methods routinely followed for semen analysis were carried out. Copper was estimated by employing atomic absorption spectrophotometry.
Results:
First split of ejaculate showed the highest number of motile sperm, the quality of which decreased from first to third. Copper level in splits 1, 2 and 3 was 29, 23 and 22 µg%, respectively.
Conclusions:
This study concluded that copper was released from throughout the genital tract.
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