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Updated: May 2, 2026
Exploring Caspase Mutations and Post-Translational Modification by Molecular Modeling Approaches
Published on: October 13, 2022
Global identification of caspase substrates using PROTOMAP (protein topography and migration analysis platform)
Melissa M Dix1, Gabriel M Simon, Benjamin F Cravatt
1Department of Chemical Physiology, The Scripps Research Institute, La Jolla, CA, USA.
Abstract:
Delineation of the natural substrate scope of proteases is important for understanding the functions of proteolytic pathways in physiology and disease. Herein we describe the protocol for PROTOMAP, a technique that combines SDS-PAGE with tandem mass spectrometry to globally identify shifts in protein migration indicative of proteolytic processing. When applied to cells undergoing apoptosis, this unbiased global method provides a snapshot of the topography and magnitude of proteolytic events associated with programmed cell death.
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