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Updated: May 2, 2026
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Leishmania metacaspase: an arginine-specific peptidase
Ricardo Martin1, Iveth Gonzalez, Nicolas Fasel
1Department of Biochemistry, University of Lausanne, Lausanne, Switzerland.
Abstract:
The purpose of this chapter is to give insights into metacaspase of Leishmania protozoan parasites as arginine-specific cysteine peptidase. The physiological role of metacaspase in Leishmania is still a matter of debate, whereas its peptidase enzymatic activity has been well characterized. Among the different possible expression systems, metacaspase-deficient yeast cells (Δyca1) have been instrumental in studying the activity of Leishmania major metacaspase (LmjMCA). Here, we describe techniques for purification of LmjMCA and its activity measurement, providing a platform for further identification of LmjMCA substrates.
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