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Related Experiment Video

Updated: May 2, 2026

Micro-dissection of Enamel Organ from Mandibular Incisor of Rats Exposed to Environmental Toxicants
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Micro-dissection of Enamel Organ from Mandibular Incisor of Rats Exposed to Environmental Toxicants

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Ameloblasts express type I collagen during amelogenesis.

N Assaraf-Weill1, B Gasse, J Silvent

  • 1UMR 7138-SAE, Research Group "Evolution & Development of the Skeleton", Université Pierre et Marie Curie, 7 quai St-Bernard, Case 5, 75005 Paris, France.

Journal of Dental Research
|February 27, 2014
PubMed
Summary
This summary is machine-generated.

This study reveals that tooth enameloid transitions to enamel in Pleurodeles waltl by altering gene expression. Ameloblasts shift from producing type I collagen (COL1A1) in enameloid to amelogenin (AMEL) in enamel.

Keywords:
Pleurodeles waltlamelogeninamphibianenameloidin situ hybridizationodontogenesis

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Area of Science:

  • Evolutionary biology
  • Developmental biology
  • Paleontology

Background:

  • Enamel and enameloid are highly mineralized vertebrate tooth tissues with distinct matrix compositions.
  • Enamel is produced by ameloblasts and rich in enamel matrix proteins (EMPs), while enameloid contains collagen fibrils and involves both odontoblasts and ameloblasts.

Purpose of the Study:

  • To investigate type I collagen (COL1A1) and amelogenin (AMEL) gene expression during enameloid and enamel formation in Pleurodeles waltl.
  • To understand the molecular mechanisms behind the enameloid-to-enamel transition during vertebrate evolution.

Main Methods:

  • Quantitative PCR (qPCR) and in situ hybridization (ISH) were used to analyze gene expression in pre- and post-metamorphic teeth.
  • Gene expression patterns of COL1A1 and AMEL were examined in ameloblasts and odontoblasts.

Main Results:

  • Ameloblasts showed weak AMEL expression during late enameloid formation, increasing significantly during enamel deposition.
  • COL1A1 transcripts were detected in ameloblasts and odontoblasts during enameloid formation but decreased and disappeared after metamorphosis.
  • A shift in ameloblast activity from COL1A1 to AMEL synthesis correlates with the transition from enameloid-rich to enamel-rich teeth.

Conclusions:

  • Pleurodeles waltl serves as a suitable model for studying the enameloid-to-enamel transition.
  • This transition likely reflects conserved evolutionary events in vertebrate tooth development.