Paraffin section light-chain immunostaining of large-cell lymphocytoma

Sixteen biopsy specimens from 11 patients with cutaneous large-cell lymphocytoma were studied by a peroxidase method using monospecific antisera to kappa and lambda Ig light chains. Five specimens had polyclonal presence of both kappa and lambda chains. Four specimens were graded as devoid of immunoreactivity, and eight were graded as non-specific because the albumin control staining was as intense as or more intense than the immunoglobulin light chains. Two specimens showing monoclonality for lambda light chains had the cells in the interfollicular spaces only, and the patients have survived for 9 and 12 years without evidence of lymphoma.