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Updated: May 2, 2026
Multiplex Detection of Bacteria in Complex Clinical and Environmental Samples using Oligonucleotide-coupled Fluorescent Microspheres
Published on: October 23, 2011
[Application of multiplex PCR for analysis of polycomponent probiotic preparations]
Aim:
Development of multiplex PCR for indication and differential analysis of CFU in a probiotic preparation containing 3 various species ofbifidobacteria (Bifidobacterium bifidum, B. longum, B. adolescentis) and 2 species of lactobacilli (Lactobacillus acidophilus, L. plantarum). MATERIALS AND METHODS; Multiplex PCR with primers to 16S RNA genes of 5 species of bacteria was applied. Samples of bacterial colonies were removed from solid nutrient medium and transferred into test-tube for multiplex PCR execution. Bacterial lysates were used as target-matrix for amplification and determination of presence of the required amplicons.
Results:
Multiplex PCR for detection of CFU of each of the bifidobacteria and lactobacilli strains in samples of 5-component probiotic with accuracy up to species was developed. A reagent kit was fine-tuned and high reproducibility and specificity of the multiplex PCR method with the accuracy of 90 - 95% of cases was shown.
Conclusion:
The multiplex PCR method developed is recommended to be applied for CFU control of each bacterial component in a complex probiotic preparation.
