A turn-on fluorescent aptasensor for adenosine detection based on split aptamers and graphene oxide

Area of Science:

• Analytical Chemistry
• Biochemistry
• Nanotechnology

Background:

• Adenosine is a crucial biomolecule involved in various physiological processes.
• Developing sensitive and selective methods for adenosine detection is important for diagnostics.
• Existing detection methods may lack sensitivity, selectivity, or simplicity.

Purpose of the Study:

• To develop a simple, sensitive, and selective turn-on fluorescent aptasensor for adenosine detection.
• To utilize the unique properties of graphene oxide and split aptamer fragments for signal amplification.

Main Methods:

• Design of split aptamer sequences that undergo target-induced conjunction.
• Utilizing graphene oxide as a quencher and signal enhancer.
• Developing a turn-on fluorescence mechanism based on aptamer-graphene oxide interactions.

Main Results:

• The developed aptasensor demonstrated high sensitivity and selectivity for adenosine detection.
• The turn-on fluorescence signal was directly correlated with adenosine concentration.
• The method showed good stability and potential for real-world applications.

Conclusions:

• The proposed fluorescent aptasensor offers a promising platform for sensitive and selective adenosine detection.
• The combination of split aptamers and graphene oxide provides an effective strategy for biosensor development.
• This approach has potential applications in biochemical analysis and disease diagnosis.